Hi,
First check all condition tables are available in your new access sequence. Second analyze the abap code of all "Requirement" related to PIR. I feel some requirement condition which is written in requirement is failing in your new access sequence.
Regards,
Sundaresan.K
Read OSS note 2307930 - Conditions not displayed in info record when pressing the conditions button
it explains the logic of condition access, so you may find the place where you missed some customizing
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