Not able to trigger Mixed loading check in sales order

Hi Experts,

I am following all the steps but not able to trigger the mixed loading check in sales order while having 2 materials and having different segregation keys.I am able to trigger another checks.

1. Schema is defined as per the standard. & HAZMAT_CHK_ACTIVE is activated.

2. DG checks are active at sales org & sales doc type with check method 2.

3. In Mixed loading check config. Specify Segregation Rules for Segregation Keys is maintained and as per that only I have maintained in the DG goods master data.

Could you guys please guide me.It is urgent.